Q345c Square column Template
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The column was washed with buffer A and the protein was eluted with buffer A containing 300 mM imidazole. The protein was further purified by size-exclusion chromatography in storage buffer (20 mM HEPES pH 7.5, 200 mM NaCl, 10 mM MgCl 2 , 10 mM KCl, 10% (v/v) glycerol) and concentrated to about 10-40 mg/ml. Aliquots were snap-frozen in liquid Q345C Square column template
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